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15 May, 16:23

You have isolated one of the genes for producing one of the blood-clotting proteins needed by some hemophiliacs. Briefly describe how you could create bacteria that would produce this protein

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  1. 15 May, 19:00
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    Isolate the plasmids of the bacterium, and use the same restriction endonuclease you have used to cut the gene for blood clotting proteins, to cut open the plasmid. Then use a ligase enzyme to join the genes with the open plasmid, to create a reccombinant plasmid. The plasmids are then taken up by the bacterium. The bacteria can multiply in a culture containing nutrients, so more genetically modified bacteria with the blood clotting genes, are produced. Or the bacteria can grow in a fermenter to produce commercial quantities of the protein
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