While testing amylase activity, John uses a buffer of pH 6 and substrate starch. He incubates them at 104ºF (40ºC). All the tubes show glucose formation, except one, which had no starch solution. Why did this happen?
A) The optimum pH for the enzyme amylase is not pH 6.
B) The optimum temperature for the enzyme amylase is not 104°F (40°C)
C) The substrate for amylase, starch, is not present.
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