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9 September, 11:31

Why does CaCl2 need to be added to cells to make them 'competent'? Why does CaCl2 need to be added to cells to make them 'competent'?

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  1. 9 September, 12:15
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    DNA is a negatively-charged molecule. Also cell membranes are negatively charged. The problem would be how to push this charges together? I f you put some CaCl2 into the mixture, the CaCl2 will "split" giving 2Cl - and Ca2+. This last ion will be attracted by the negatively charged DNA and will "cover" it, hiding its negative charge. Hope this answers the question.
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