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16 July, 14:18

Which statements are true if the particle size in a packed column used in HPLC is decreased?

a. increased resolution

b. increased separation

c. efficiency allows the use of a lower operating pressure

d. decreased operating temperatures requires the use of a nonpolar mobile phase

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  1. 16 July, 15:52
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    b. increased separation

    a. increased resolution

    Explanation:

    As the column particle size is decreased, the plate height will decrease.

    And as plate height decreases separation efficiency will increases.
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