A solution of a specific vitamin has? max = 275 nm and a concentration of 2.71 * 10-7 M. The absorbance of the solution at 275 nm is A = 0.150. What is the molar absorptivity of the vitamin at 275 nm? (sample pathlength = 1.00 cm)

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Chemistry

Rey Meza

22 November, 22:49

A solution of a specific vitamin has? max = 275 nm and a concentration of 2.71 * 10-7 M. The absorbance of the solution at 275 nm is A = 0.150. What is the molar absorptivity of the vitamin at 275 nm? (sample pathlength = 1.00 cm)

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Zariah Callahan · Answer 1

To solve this problem, we use the Beer-Lambert's Law this relates absorbance and concentration of the species that is absorbing light. It is expressed as A = eCl where e is the molar absorptivity, C is the concentration and l is the path length.

A = eCl

0.150 = e (2.71x10^-7 M) (1.00 cm)

e = 553505.5351 L / mol-cm