A researcher is performing a gram stain on a specimen in a medical laboratory. She gets distracted by a phone call and accidentally uses water instead of alcohol to decolorize. What may happen as a result of this mistake?

Question

Chemistry

Kody Callahan

26 August, 15:33

A researcher is performing a gram stain on a specimen in a medical laboratory. She gets distracted by a phone call and accidentally uses water instead of alcohol to decolorize. What may happen as a result of this mistake?

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Answers (2)

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Beatrice Gonzales · Answer 1

It wouldn't be able to separate negative gram from positive. it would not wash away some of the crystal violet stain effectively. Gram - Cells will appear to be the same hue. Water can also dilute cells or wash them off the slide

Kayla Velez · Answer 2

Would not be able to differentiate gram - from + it would not efficiently wash some of the crystal violet stain away. gram - cells will still remain the same color. also water may dilute or wash cells off the slide