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16 November, 14:37

When performing qualitative tests in glass test tubes, such as the iodoform test or dinitrophenylhydrazine test, why should you avoid rinsing the glassware with acetone prior this experiment?

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  1. 16 November, 17:38
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    It will lead to false positive (or negative results) for this classification tests.

    Explanation:

    In this tests, the functional group that is really being tested for is the Carbonyl group. In the iodoform, the presence of a Carbonyl group gives a reaction and in the dinitrophenylhydrazine test, aldehydes give a reaction and ketones do not.

    So, rinsing the glassware with acetone is introducing ketone before the qualitative test has even began, thereby leading to false results for each of the two tests mentioned in the question.
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