DNP is referred to as an electron transport chain (ETC) "decoupler," because it uncouples the oxidation of NADH from the synthesis of ATP. In the presence of DNP, NADH is still oxidized by complex I and oxygen can be reduced, but no ATP is produced. Cyanide, a different metabolic poison, inhibits ATP production through a different mechanism - it inhibits the oxidation of complex IV by oxygen. Would you consider cyanide an ETC decoupler?
No, because oxidation of NADH by complex I still occurs in the presence of cyanide.
Yes, because cyanide disrupts the hydrogen ion gradient in mitochondria.
No, because oxidation of NADH is blocked in the presence of cyanide.
Yes, because cyanide inhibits ATP production in mitochondria.
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