Alpha-mannosidase follows Michaelis-Menten kinetics. A 3 micromolar solution of alpha-mannosidase catalyzes the formation of. 66 moles of p nitrophenol is-1 when incubated with the substrate 4-nitrophenyl alpha D-mannoside at a concentration 130 times greater than the Km for that substrate. What is alpha mannosidase Km

Question

Chemistry

Lamar Lloyd

31 March, 05:33

Alpha-mannosidase follows Michaelis-Menten kinetics. A 3 micromolar solution of alpha-mannosidase catalyzes the formation of. 66 moles of p nitrophenol is-1 when incubated with the substrate 4-nitrophenyl alpha D-mannoside at a concentration 130 times greater than the Km for that substrate. What is alpha mannosidase Km

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Aylin Levy · Answer 1

220

Explanation:

V₀ = Vmax (D) / (D) + km

0.66/100=vmax (130km) / (130km) + (km)

Vmax = 0.66*131/130*1000 = 0.6650mm/d

kwat = vmax/ET

=0.665*10⁻³ / 3*10⁻⁶

= 0.22*10³

= 220