The concentration of O-Nitro Phenol is proportional to

Select one:

a. the concentration of b-galactosidase

b. the concentration of Luria Broth

c. the concentation of glucose

d. the concentration of lactose

e. the concentration of ONPG

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a. The concentration of β-galactosidase

Explanation:

β-Galactosidase is an enzyme that hydrolyzes ß-D-galactosides like lactose to glucose and galactose.

It also cleaves o-nitrophenyl-β-D-galactoside (ONPG) to yield galactose and o-nitrophenol, which is yellow, and can be measured by its absorption at 420 nm.

If the concentration of ONPG is greater than that of β-galactosidase, the concentration of o-nitrophenol produced is proportional to the concentration of the enzyme.