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3 February, 04:58

A student has an unknown sample of solution X. This solution is placed in a 1.00 cm wide cuvet and inserted into the spectrometer, producing an absorbance reading of 0.275 at a wavelength of 525.0 nm. What is the concentration of solution X in the unknown sample

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  1. 3 February, 08:08
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    The concentration of the sample is 3.564x10⁻³M.

    Explanation:

    Using Lambert-Beer law, absorbance of a sample is directely proportional to its concentration.

    The general graph of the absorbance of the standards with different concentrations is:

    Y = 75.9X + 0.0045

    R² = 0.9946

    Where Y is the absorbance of the sample and X its concentration in mole/L.

    If a solution has an absorbance of 0.275:

    0.275 = 75.9X + 0.0045

    0.2705 = 75.9X

    3.564x10⁻³M = X → The concentration of the sample.
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