The Galapagos Islands were the site of

an outpost of the Incas.

Charles Darwin's study that resulted in the theory of evolution.

Christopher Columbus's second landing in the Americas.

Ecuador's largest volcanic eruption

Charles Darwin's study that resulted in the theory of evolution.

Explanation:

Galapagos Island is a volcanic enclave in the Pacific Oceans. The island is celebrated for its variety of species that it contains. The island has been a place of the visit after Charles Darwin set a foot on it in 1835.

The island has been a site of volcanic mountains, giant tortoise, national parks, etc. Apart from it the site is also popularly known for the evolution of the theory of natural selection by Charles Darwin. It is on this island Charles Darwin studied the species and developed the theory of natural selection.

Thus the correct answer is the second option.