How did US annexation of West Florida impact Louisiana?

A It ignited disputes between Creoles and settlers from West Florida

B It redefined borders as West Florida became the eastern part of Louisiana.

C It created disputes between the two territories over land and political boundaries

D It led to an influx of refugees, including free people of color, and slaves.

The US annexation of impacted Louisiana as it created disputes between the two territories over land and political boundaries. (C)

Explanation:

US gained control over the commerce over Mississippi river by acquiring Louisiana from France. James Madison, the then president, further went to expand America's boundaries and wanted to acquire west Florida for this.

From time to time, the powers of Louisiana have passed over in different hands (France, US and Spain). Because of uncertainties in geographical and territorial boundaries in Louisiana, the US claimed the Missouri river, Gulf coast and Mississippi river in its interest.

After Louisiana Purchase treaty, US also claimed that West Florida was under this treaty. Spain denied this Claim. USA was also intruding in Louisiana which was unacceptable to Creole residents. All these caused problems in Louisiana.