What was the main impact of the anti Federalist on the adoption of the us constitution

The most signficant impact of the anti-federalists on the Constitution was the implementation of the Bill of Rights.

Anti-federalists were concerned that the new US Constitution gave too much power to the federal government. Anti-federalists argued that a strong central (aka federal) government would result in tyranny, just like it did when America was still part of the British empire. This is why the anti-federalists wanted a list of rights that all American citizens can possess. This list of rights would check the power of the federal government and prevent the government from restricting the rights of citizens.