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29 March, 20:03

How to calculate concentration with just the absorbance and % transmittance measurements from a spectrometer?

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  1. 29 March, 21:07
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    A=ε*l*c

    A = 2 - log₁₀ % T

    Step-by-step explanation:

    There is a linear relationship between the concentration of a sample and absorbance according to Beer-Lambert Law.

    A=ε*l*c

    where;

    A=absorbance

    ε=absorption coefficient

    l=path length

    c=concentration

    Because % transmittance is transmittance value multiplied by 100 then, the equation that will allow us calculate absorbance from % transmittance value will be;

    A = 2 - log₁₀ % T where T is transmittance.
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