Ask Question
12 November, 08:09

After running a SDS-PAGE assay on an unknown protein with molecular size 180 kD, you find that the protein shows up as two bands on the polyacrylamide gel which correlate with a 30 kD subunit and a 50 kD subunit. What is a possible ratio of 30 kD subunits to 50 kD subunits that could constitute this mystery protein?

+3
Answers (1)
  1. 12 November, 08:41
    0
    One 30 kD subunit: Three 50 kD subunits

    Explanation:

    SDS-PAGE (sodium dodecyl sulfate-polyacrylamide gel electrophoresis) is a variant of electrophoresis, which is used in the analysis of molecules (proteins) on the basis os size.

    Sodium dodecyl sulfate (SDS) is an ionic detergent that denatures proteins and make them uniformly charged.

    In this process proteins are separated on the basis of size, not on the basis of charge, and recognized by band size.

    After running a SDS-PAGE assay on an unknown protein with molecular size 180 kD proteins show two bands on the basis of size, these are of 30 kD subunit, and three 50 kD subunits,
Know the Answer?
Not Sure About the Answer?
Find an answer to your question ✅ “After running a SDS-PAGE assay on an unknown protein with molecular size 180 kD, you find that the protein shows up as two bands on the ...” in 📘 Biology if you're in doubt about the correctness of the answers or there's no answer, then try to use the smart search and find answers to the similar questions.
Search for Other Answers