You have genetically engineered green fluorescent protein (GFP) containing a KDEL sequence (GFP-KDEL). When GFP-KDEL is expressed in normal human fibroblasts and examined using fluorescence microscopy, the fluorescence appears diffuse across the cytoplasm. How would you explain this observations given that KDEL is supposed to be an ER-specific sorting sequence

Question

Biology

Abbie Knox

16 November, 11:46

You have genetically engineered green fluorescent protein (GFP) containing a KDEL sequence (GFP-KDEL). When GFP-KDEL is expressed in normal human fibroblasts and examined using fluorescence microscopy, the fluorescence appears diffuse across the cytoplasm. How would you explain this observations given that KDEL is supposed to be an ER-specific sorting sequence

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Atkins · Answer 1

KDEL In ER Sequencing

Explanation:

KDEL is assume to be in the ER, in any case, since the inquiry says a GFP joins the succession, the cell would not have the option to peruse the grouping, accordingly blending the GFP in cytosolic ribosomes If the GFP is melded to the C-end of Sec62 that outcomes a cytosolic limitation for GFP, however Sec62 is a basic film protein (~40 kDa) not only a sign arrangement. The Sec62's TMs are situated on the N-end I am uncertain about whether it works or not but rather you can attempt to shorten the C-end to lessen the size on the off chance that it is an issue Hence, the right answer for observation is "KDEL is support the sequencing of ER - specifing Sorting"