You isolate intact mitochondria and equilibrate them in a buffered solution at pH 9, containing 0.1 M KCl and ADP plus Pi, but without succinate. You collect them by centrifugation, and quickly resuspend them in a new buffer at pH 7, without KCl, but with valinomycin (a K ionophore). Note: the K rushing out will create a huge positive charge differential. What do you predict will be the result on oxygen consumption and the production of ATP?

Question

Biology

Eugene Pruitt

27 July, 10:49

You isolate intact mitochondria and equilibrate them in a buffered solution at pH 9, containing 0.1 M KCl and ADP plus Pi, but without succinate. You collect them by centrifugation, and quickly resuspend them in a new buffer at pH 7, without KCl, but with valinomycin (a K ionophore). Note: the K rushing out will create a huge positive charge differential. What do you predict will be the result on oxygen consumption and the production of ATP?

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Chaz Greene · Answer 1

Explanation : the amount of ATP generated per unit of oxygen consumed can vary significantly.

the rate of aerobic metabolism, requires careful interpretation with respect to the amount of ATP produced.