You are interpreting data on a DNA chip or microarray. You expose the chip to a mixture of two cDNA populations: one from cells that were not treated with a glucocorticoid hormone (untreated controls; labeled with a red fluorescent dye) and one from cells that were treated with glucocorticoid hormones (glucocorticoid-treated; labeled with green fluorescent dye). You look at a spot on the chip representing the gene for glutamyltransferase and it is green. How do you interpret this result?

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Biology

Barajas

13 December, 00:38

You are interpreting data on a DNA chip or microarray. You expose the chip to a mixture of two cDNA populations: one from cells that were not treated with a glucocorticoid hormone (untreated controls; labeled with a red fluorescent dye) and one from cells that were treated with glucocorticoid hormones (glucocorticoid-treated; labeled with green fluorescent dye). You look at a spot on the chip representing the gene for glutamyltransferase and it is green. How do you interpret this result?

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Sammy Lee · Answer 1

DNA microarray is a technique used to measure the level of the different expression of the large number of genes. The DNA micro array contains the DNA spots known as probes.

The glutamyltransferase gene is green and cannot be expressed in the control cDNA population. This gene is expressable in the population that are treated with glucocorticoid.