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5 September, 08:33

Match the following items,

1. obtaining DNA for analysis from blood or other body fluid

quantification

using heat or chemicals to break open the cells and free the

DNA

purification of the sample

separation of cellular debris and other impurities from the

DNA

gel electrophoresis to separate

DNA fragments

sample collection

4. determination if the sample is sufficient for analysis

5. copying the DNA using the polymerase chain reaction

DNA extraction

6. electrically ordering the fragments

amplification using PCR

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Answers (1)
  1. 5 September, 10:16
    0
    1. Obtaining DNA for analysis from blood or other body fluid - sample collection.

    Using heat or chemicals to break open the cells and free the DNA - DNA extraction.

    Separation of cellular debris and other impurities from the DNA - purification of the sample

    4. Determination if the sample is sufficient for analysis - - quantification

    5. Copying the DNA using the polymerase chain reaction- - electrically ordering the fragments amplification using PCR

    Explanation:

    These are the processes involve in extraction and amplification of DNA sample.

    Quantification is done to know if the sample is enough for the whole process of DNA extraction to occur.

    DNA sample can be obtained from blood or other body fluids as viscera or semen.

    The DNA from the sample is extracted and make free from other cellular components. The lysis of the cell takes place by heat to let the nucleus broken and free the DNA in the cell.

    Precipitation: The DNA is purified and precipitated in the ethanol solution as it is insoluble in ethanol.

    By gel electrophoresis the fragments of DNA obtained will be sequenced.

    The DNA obtained is amplified using polymerase chain reaction.
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