What situation best represents how DNA sequencing, PCR, and gene probes might be used together? Multiple Choice Scientists might replicate a strand of DNA using PCR before sequencing it. Once the sequence is known, they can produce a corresponding gene probe. Scientists might sequence a strand of DNA before replicating it with PCR. Once it is replicated, they can create a complementary gene probe. Scientists might create a gene probe to find the correct DNA strand to sequence. Once it is sequenced, they might replicate it with PCR.

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Biology

Madison Sweeney

25 May, 10:53

What situation best represents how DNA sequencing, PCR, and gene probes might be used together? Multiple Choice Scientists might replicate a strand of DNA using PCR before sequencing it. Once the sequence is known, they can produce a corresponding gene probe. Scientists might sequence a strand of DNA before replicating it with PCR. Once it is replicated, they can create a complementary gene probe. Scientists might create a gene probe to find the correct DNA strand to sequence. Once it is sequenced, they might replicate it with PCR.

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Ali Peterson · Answer 1

Scientists might replicate a strand of DNA using PCR before sequencing it. Once the sequence is known, they can produce a corresponding gene probe

Explanation:

PCR refers to the polymerase chain reaction that amplifies the small sample of DNA into multiple copies in three steps. These steps are denaturation of sample DNA to produce single-stranded template strand, binding of primer to the template and elongation. The multiple copies of the sample DNA are then used to decipher its sequence using various sequencing methods.

Once the sequence of the sample DNA is known, the short, single-stranded DNA molecules that are complementary to the specific sequence of DNA are formed. These single-stranded DNA molecules are called DNA probe and are used to detect the specific nucleotide sequence in some other sample DNA.