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11 February, 20:55

A calibration curve was created to determine the quantity of protein in a solution. The calibration curve has the form of a straight line with the equation A = 0.0182 x + 0.007 where A is the corrected absorbance of the solution and x is quantity of protein in the solution in units of micrograms (μg). Determine the quantity of protein in a solution that has an absorbance of 0.234. A blank solution has an absorbance of 0.055.

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  1. 12 February, 00:27
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    12.5 µg

    Explanation:

    We are given the absorbance from the calibration curve:

    A = 0.0182 x + 0.007

    A = 0.234 (It is already corrected, do not need the blank value)

    so we can solve for x, the quantity of protein

    x = (0.234 - 0.007) µg / 0.0182 = 12.5 µg

    Note we are given the value for a blank solution but we do not need it for the absorbance of our sample since it is already corrected as stated in the question. If it were not a corrected absorbance, we will need to correct the absorbance value. The absorbance of the blank is caused by some absorption from the solvent, cuvette, internal reflection, etc.
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