If you are trying to pipet an unknown liquid with a pipetman and the liquid keeps running out of the tip before you can transfer it, what are two possible reasons for this? What can you do to remedy the situation?

Question

Chemistry

Alfred Salas

10 December, 13:05

If you are trying to pipet an unknown liquid with a pipetman and the liquid keeps running out of the tip before you can transfer it, what are two possible reasons for this? What can you do to remedy the situation?

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Answers (1)

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Nigel Friedman · Answer 1

This is due to the physical properties of the sample, since it affects the volume dispensed.

Explanation:

For example, in the case of very dense samples, selected samples to adhere to the surface of the tip, dispensing more slowly. In contrast, ethanol samples are less viscous and more volatile and are dispensed more rapidly. Some of the ways to minimize these inconveniences are the use of ultra low retention pipette tips, since they have a hydrophobic plastic additive that prevents the liquid from adhering to the inside of the tip.

Another way is to use the reverse pipetting.