During the GFP purification experiment, the instructor will have to make breaking buffer for the students to use. This buffer contains 150mM NaCl. Given a bottle of crystalline NaCl (M. W. = 40g/mol), describe how you would make 500ml of 150mM NaCl. Include the type and sizes of any measuring vessels that you use to make the solution.

Question

Chemistry

String Bean

11 December, 04:07

During the GFP purification experiment, the instructor will have to make breaking buffer for the students to use. This buffer contains 150mM NaCl. Given a bottle of crystalline NaCl (M. W. = 40g/mol), describe how you would make 500ml of 150mM NaCl. Include the type and sizes of any measuring vessels that you use to make the solution.

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Simon Herrera · Answer 1

150 mM NaCl = 0.15 M NaCl

500 ml of 0.15 M NaCl = 500 x 0.15 ml of M NaCl

= 75 ml of M NaCl

M. W = 40 g

1000 ml of M NaCl requires 40 g

75 ml of M NaCl requires (40/1000) x75

= 3 g

We shall take 500 ml of water in a graduated cylinder. Then we weigh 3 g of salt and add it to 500 ml of water and mix it. We shall get 500 ml of 150mM NaCl.